Review





Similar Products

mdck type ii cells  (ATCC)
96
ATCC mdck type ii cells
Mdck Type Ii Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mdck+(type+ii)+cells/MDCK%2E2/pm41576074-315-0-4
Average 96 stars, based on 1 article reviews
mdck type ii cells - by Bioz Stars, 2026-09
96/100 stars
  Buy from Supplier
type ii alveolar epithelial cells  (ATCC)
95
ATCC type ii alveolar epithelial cells
Effect of hsa-miR-4634 on interferon expression in <t>epithelial</t> cells. ( A ) A549 cells were infected at MOI 0.5, 1.0, and 3.0, and the percentage of HMPV-positive cells and cell death was quantified by flow cytometry. ( B ) A549 cells were infected with HMPV at an MOI of 3.0 for 24 h, and the expression of hsa-miR-4634 was assessed by RT-qPCR. ( C – E ) A549 cells were transfected by electroporation with mimic-4634 (100 nM) or inhibitor-4634 (50 nM). After 24 h of culture, cells were infected with HMPV at an MOI of 3.0 for 24 h. Total RNA was extracted to assess the expression of IFN-α2, IFN-β, and IFN-λ2/3 by qPCR. Bar graphs represent mean of three independent experiments ± SEM. Statistical differences were calculated using the Student t -test ( B ) or ANOVA followed by Sidak’s multiple comparison test ( A , C – E ). * p < 0.05; ** p < 0.01, *** p < 0.001, **** p ≤ 0.0001.
Type Ii Alveolar Epithelial Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mdck+(type+ii)+cells/MDCK%2ESTAT1KO%3B+CRISPR%2FCas9%3B+Mod%2E+Cell+Line%3B+Dog+kidney/pmc10675405-36-14-19
Average 95 stars, based on 1 article reviews
type ii alveolar epithelial cells - by Bioz Stars, 2026-09
95/100 stars
  Buy from Supplier
madin-darby canine kidney type ii (mdck ii) cells ecacc 00062107  (European Collection of Authenticated Cell Cultures)
90
European Collection of Authenticated Cell Cultures madin-darby canine kidney type ii (mdck ii) cells ecacc 00062107
Effect of hsa-miR-4634 on interferon expression in <t>epithelial</t> cells. ( A ) A549 cells were infected at MOI 0.5, 1.0, and 3.0, and the percentage of HMPV-positive cells and cell death was quantified by flow cytometry. ( B ) A549 cells were infected with HMPV at an MOI of 3.0 for 24 h, and the expression of hsa-miR-4634 was assessed by RT-qPCR. ( C – E ) A549 cells were transfected by electroporation with mimic-4634 (100 nM) or inhibitor-4634 (50 nM). After 24 h of culture, cells were infected with HMPV at an MOI of 3.0 for 24 h. Total RNA was extracted to assess the expression of IFN-α2, IFN-β, and IFN-λ2/3 by qPCR. Bar graphs represent mean of three independent experiments ± SEM. Statistical differences were calculated using the Student t -test ( B ) or ANOVA followed by Sidak’s multiple comparison test ( A , C – E ). * p < 0.05; ** p < 0.01, *** p < 0.001, **** p ≤ 0.0001.
Madin Darby Canine Kidney Type Ii (Mdck Ii) Cells Ecacc 00062107, supplied by European Collection of Authenticated Cell Cultures, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mdck+(type+ii)+cells/madin+darby+canine+kidney++mdck++cells/pm37766238-47-0-10
Average 90 stars, based on 1 article reviews
madin-darby canine kidney type ii (mdck ii) cells ecacc 00062107 - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
madin–darby canine kidney (mdck) type ii cells  (Thermo Fisher)
90
Thermo Fisher madin–darby canine kidney (mdck) type ii cells
Effect of hsa-miR-4634 on interferon expression in <t>epithelial</t> cells. ( A ) A549 cells were infected at MOI 0.5, 1.0, and 3.0, and the percentage of HMPV-positive cells and cell death was quantified by flow cytometry. ( B ) A549 cells were infected with HMPV at an MOI of 3.0 for 24 h, and the expression of hsa-miR-4634 was assessed by RT-qPCR. ( C – E ) A549 cells were transfected by electroporation with mimic-4634 (100 nM) or inhibitor-4634 (50 nM). After 24 h of culture, cells were infected with HMPV at an MOI of 3.0 for 24 h. Total RNA was extracted to assess the expression of IFN-α2, IFN-β, and IFN-λ2/3 by qPCR. Bar graphs represent mean of three independent experiments ± SEM. Statistical differences were calculated using the Student t -test ( B ) or ANOVA followed by Sidak’s multiple comparison test ( A , C – E ). * p < 0.05; ** p < 0.01, *** p < 0.001, **** p ≤ 0.0001.
Madin–Darby Canine Kidney (Mdck) Type Ii Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mdck+(type+ii)+cells/madin+darby+canine+kidney++mdck++cells/10__1091_slash_mbc__e22___09___0448-198-0-13
Average 90 stars, based on 1 article reviews
madin–darby canine kidney (mdck) type ii cells - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
mdck ii american type culture collection cells  (ATCC)
99
ATCC mdck ii american type culture collection cells
Effect of hsa-miR-4634 on interferon expression in <t>epithelial</t> cells. ( A ) A549 cells were infected at MOI 0.5, 1.0, and 3.0, and the percentage of HMPV-positive cells and cell death was quantified by flow cytometry. ( B ) A549 cells were infected with HMPV at an MOI of 3.0 for 24 h, and the expression of hsa-miR-4634 was assessed by RT-qPCR. ( C – E ) A549 cells were transfected by electroporation with mimic-4634 (100 nM) or inhibitor-4634 (50 nM). After 24 h of culture, cells were infected with HMPV at an MOI of 3.0 for 24 h. Total RNA was extracted to assess the expression of IFN-α2, IFN-β, and IFN-λ2/3 by qPCR. Bar graphs represent mean of three independent experiments ± SEM. Statistical differences were calculated using the Student t -test ( B ) or ANOVA followed by Sidak’s multiple comparison test ( A , C – E ). * p < 0.05; ** p < 0.01, *** p < 0.001, **** p ≤ 0.0001.
Mdck Ii American Type Culture Collection Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mdck+(type+ii)+cells/MDCK/pm33794243-65-0-2
Average 99 stars, based on 1 article reviews
mdck ii american type culture collection cells - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier
mdck type ii cell lines  (ATCC)
99
ATCC mdck type ii cell lines
Effect of hsa-miR-4634 on interferon expression in <t>epithelial</t> cells. ( A ) A549 cells were infected at MOI 0.5, 1.0, and 3.0, and the percentage of HMPV-positive cells and cell death was quantified by flow cytometry. ( B ) A549 cells were infected with HMPV at an MOI of 3.0 for 24 h, and the expression of hsa-miR-4634 was assessed by RT-qPCR. ( C – E ) A549 cells were transfected by electroporation with mimic-4634 (100 nM) or inhibitor-4634 (50 nM). After 24 h of culture, cells were infected with HMPV at an MOI of 3.0 for 24 h. Total RNA was extracted to assess the expression of IFN-α2, IFN-β, and IFN-λ2/3 by qPCR. Bar graphs represent mean of three independent experiments ± SEM. Statistical differences were calculated using the Student t -test ( B ) or ANOVA followed by Sidak’s multiple comparison test ( A , C – E ). * p < 0.05; ** p < 0.01, *** p < 0.001, **** p ≤ 0.0001.
Mdck Type Ii Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mdck+(type+ii)+cells/MDCK/pm30928021-113-3-8
Average 99 stars, based on 1 article reviews
mdck type ii cell lines - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier
madine darby canine kidney type ii mdck ii cells  (ATCC)
99
ATCC madine darby canine kidney type ii mdck ii cells
Effect of hsa-miR-4634 on interferon expression in <t>epithelial</t> cells. ( A ) A549 cells were infected at MOI 0.5, 1.0, and 3.0, and the percentage of HMPV-positive cells and cell death was quantified by flow cytometry. ( B ) A549 cells were infected with HMPV at an MOI of 3.0 for 24 h, and the expression of hsa-miR-4634 was assessed by RT-qPCR. ( C – E ) A549 cells were transfected by electroporation with mimic-4634 (100 nM) or inhibitor-4634 (50 nM). After 24 h of culture, cells were infected with HMPV at an MOI of 3.0 for 24 h. Total RNA was extracted to assess the expression of IFN-α2, IFN-β, and IFN-λ2/3 by qPCR. Bar graphs represent mean of three independent experiments ± SEM. Statistical differences were calculated using the Student t -test ( B ) or ANOVA followed by Sidak’s multiple comparison test ( A , C – E ). * p < 0.05; ** p < 0.01, *** p < 0.001, **** p ≤ 0.0001.
Madine Darby Canine Kidney Type Ii Mdck Ii Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mdck+(type+ii)+cells/MDCK/pmc07070169-88-1-17
Average 99 stars, based on 1 article reviews
madine darby canine kidney type ii mdck ii cells - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier

Image Search Results


Effect of hsa-miR-4634 on interferon expression in epithelial cells. ( A ) A549 cells were infected at MOI 0.5, 1.0, and 3.0, and the percentage of HMPV-positive cells and cell death was quantified by flow cytometry. ( B ) A549 cells were infected with HMPV at an MOI of 3.0 for 24 h, and the expression of hsa-miR-4634 was assessed by RT-qPCR. ( C – E ) A549 cells were transfected by electroporation with mimic-4634 (100 nM) or inhibitor-4634 (50 nM). After 24 h of culture, cells were infected with HMPV at an MOI of 3.0 for 24 h. Total RNA was extracted to assess the expression of IFN-α2, IFN-β, and IFN-λ2/3 by qPCR. Bar graphs represent mean of three independent experiments ± SEM. Statistical differences were calculated using the Student t -test ( B ) or ANOVA followed by Sidak’s multiple comparison test ( A , C – E ). * p < 0.05; ** p < 0.01, *** p < 0.001, **** p ≤ 0.0001.

Journal: Viruses

Article Title: Human Metapneumovirus-Induced Host microRNA Expression Impairs the Interferon Response in Macrophages and Epithelial Cells

doi: 10.3390/v15112272

Figure Lengend Snippet: Effect of hsa-miR-4634 on interferon expression in epithelial cells. ( A ) A549 cells were infected at MOI 0.5, 1.0, and 3.0, and the percentage of HMPV-positive cells and cell death was quantified by flow cytometry. ( B ) A549 cells were infected with HMPV at an MOI of 3.0 for 24 h, and the expression of hsa-miR-4634 was assessed by RT-qPCR. ( C – E ) A549 cells were transfected by electroporation with mimic-4634 (100 nM) or inhibitor-4634 (50 nM). After 24 h of culture, cells were infected with HMPV at an MOI of 3.0 for 24 h. Total RNA was extracted to assess the expression of IFN-α2, IFN-β, and IFN-λ2/3 by qPCR. Bar graphs represent mean of three independent experiments ± SEM. Statistical differences were calculated using the Student t -test ( B ) or ANOVA followed by Sidak’s multiple comparison test ( A , C – E ). * p < 0.05; ** p < 0.01, *** p < 0.001, **** p ≤ 0.0001.

Article Snippet: A549 cells, a human alveolar basal epithelial cell line derived from lung adenocarcinoma with type II alveolar epithelial cells (ATCC CCL-185C, Manassas, VA, USA), were cultivated in F12K medium (Gibco, Gaithersburg, MD, USA) supplemented with 10% FBS (Gibco, Gaithersburg, MD, USA) and 1% penicillin-streptomycin (Gibco, Gaithersburg, MD, USA), herein referred as the complete medium.

Techniques: Expressing, Infection, Flow Cytometry, Quantitative RT-PCR, Transfection, Electroporation, Comparison

Effect of hsa-miR-4634 on viral replication in macrophages and epithelial cells. Viral copies of the HMPV N gene were quantified by RT-qPCR. Cells were transfected by electroporation with mimic (100 nM) and inhibitor (50 nM). ( A ) Macrophages and ( B ) epithelial cells were infected for 24 h with HMPV at MOI 1 or MOI 3, respectively. Bar graphs represent the mean of two independent experiments with similar results ± SEM. Statistical differences were calculated using the Student t -test. * p < 0.05, *** p <0.001.

Journal: Viruses

Article Title: Human Metapneumovirus-Induced Host microRNA Expression Impairs the Interferon Response in Macrophages and Epithelial Cells

doi: 10.3390/v15112272

Figure Lengend Snippet: Effect of hsa-miR-4634 on viral replication in macrophages and epithelial cells. Viral copies of the HMPV N gene were quantified by RT-qPCR. Cells were transfected by electroporation with mimic (100 nM) and inhibitor (50 nM). ( A ) Macrophages and ( B ) epithelial cells were infected for 24 h with HMPV at MOI 1 or MOI 3, respectively. Bar graphs represent the mean of two independent experiments with similar results ± SEM. Statistical differences were calculated using the Student t -test. * p < 0.05, *** p <0.001.

Article Snippet: A549 cells, a human alveolar basal epithelial cell line derived from lung adenocarcinoma with type II alveolar epithelial cells (ATCC CCL-185C, Manassas, VA, USA), were cultivated in F12K medium (Gibco, Gaithersburg, MD, USA) supplemented with 10% FBS (Gibco, Gaithersburg, MD, USA) and 1% penicillin-streptomycin (Gibco, Gaithersburg, MD, USA), herein referred as the complete medium.

Techniques: Quantitative RT-PCR, Transfection, Electroporation, Infection